RNA

Part:BBa_K3258043:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


MGA (Malachite Green Aptamer) Scaffold


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to maximize fluorescent output of the MGA RNA aptamer by repetition of base unit, while also maintaining synthesizability of the construct via Twist Biosciences.


Source

Synthetic construct.

References